Here we show that migration of cardiomyocytes to the injury site is essential for heart regeneration.
Furthermore, many parameters associated with adult neurogenesis are altered in models of Alzheimer’s and other neurodegenerative diseases, and in models of ischemia or schizophrenia.
Albert AU - Caron, Sophie J CAU - Schier, Alexander F.
PY - 2012/1/1Y1 - 2012/1/1N2 - This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish.
Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth.
When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate.
It is therefore critical that the developmental timecourse of new neurons be mapped out, so we know when new neurons become functionally relevant, or whether they might even have different functions at different ages.