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Birthdating neurons

birthdating neurons-36

Here we show that migration of cardiomyocytes to the injury site is essential for heart regeneration.

Medscape uses cookies to customize the site based on the information we collect at registration.This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish.The methods used are birthdating analysis by photoconverted fluorescent protein tracing in vivo (BAPTI) and BAPTI combined with subpopulation markers (BAPTISM).Increasingly, evidence suggests that newborn neurons might be involved in hippocampal functions that are particularly dependent on the dentate gyrus, such as pattern separation.Furthermore, newborn neurons at different maturation stages may make distinct contributions to learning and memory.Adult brain neurogenesis is closely linked with learning and memory, and it has also been implicated in anxiety and depression (for recent reviews, see Aimone et al., 2010; Deng et al., 2010).

Furthermore, many parameters associated with adult neurogenesis are altered in models of Alzheimer’s and other neurodegenerative diseases, and in models of ischemia or schizophrenia.

Albert AU - Caron, Sophie J CAU - Schier, Alexander F.

PY - 2012/1/1Y1 - 2012/1/1N2 - This protocol describes how the photoconvertible protein Kaede can be used to determine the birthdates of neurons in live zebrafish.

Because Kaede can be converted from green to red fluorescence at any developmental time point, it serves as a temporal landmark for cell birth.

When it is used in combination with subpopulation markers, the eventual fate of a cell can be correlated with its birthdate.

It is therefore critical that the developmental timecourse of new neurons be mapped out, so we know when new neurons become functionally relevant, or whether they might even have different functions at different ages.